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KMID : 0880220070450010048
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Journal of Microbiology
2007 Volume.45 No. 1 p.48 ~ p.52
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Development of a Virus Concentration Method and its Application for the Detection of Noroviruses in Drinking Water in China
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Liu Junyi
Wu Qingping
Kou Xiaoxia
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Abstract
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A new procedure for the concentration of nonoviruses from water samples has been developed. This procedure (calcium flocculation-citrate dissolution method) uses the following steps: virus flocculation formed by treatment with 1 M CaCl2 and 1 M Na2HPO4, virus release by sodium citrate dissolution (0.3 M Na citrate, pH 3.5), and virus re-concentration by ultrafiltration. When reverse transcription (RT)-PCR was performed after the procedure, the overall detection sensitivity for seeded noroviruses in a one liter drinking water sample was as low as 1 RT-PCR unit, which is equal to a 10-6 dilution of the virus sample. This approach showed at least a 5-fold-higher sensitivity than the current method with its three steps of adsorption-elution-concentration. The newly developed procedure was used to test different brands of bottled drinking water from China for putative contamination with noroviruses. A total of 144 samples were analyzed; all of the samples were negative for norovirus specific nucleic acids.
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KEYWORD
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Noroviruses, concentration, drinking water
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